| | |
| NCCBNr | 3451 |
| Name | M13KO7 |
| Element type | Phage |
| Collection nrs | PC-V3451 |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Vieira, J., Messing, J., Meth.Enzymol.(1987) 153: 3-11 [PMID: 3323803]
Sambrook, J. et al., Molecular Cloning (1989) 1: 4.44 |
| Constructed from | M13 |
| Host propagation | Escherichia coli (F+, F'or Hfr) |
| Remarks propag./selection | M13KO7 carries gene II* (from M13mp1) and a plasmid ori derived from p15A, the mutated gene II product interacts less efficiently with it's own phage ori than with the phagemid ori of replication |
| Virus morphology | filamentous |
| Virus size | 8.7 kb |
| Lysogenic | No |
| Plaque forming | Yes |
| Plaque morphology | No |
| Receptor | F pili |
| Nucleic acid type | DNA, single, circular |
| Start codon present | No |
| Mobility | No |
| Resistance | Km |
| Usage | isolation of ssDNA; M13KO7 preferentially packages plasmid over phage DNA; interference resistant |
| Medium | Luria Broth |
| Supply | LS |
| PriceCode | 1 |