| NCCBNr | 3436 |
| Name | pBBR1MCS-4 |
| Element type | Plasmid |
| Collection nrs | PC-V3436 |
| Date of accession | Jan 1996 |
| Deposited by | M.E. Kovach |
| Distributed in | Escherichia coli K12 DH5alpha |
| Distribution restricted | Yes |
| Natural plasmid | No |
| Literature | Kovach et al., Biotechniques (1994) 5:800-802 [PMID: 8068328] Kovach, M.E. et al., Gene (1995) 166:175-176 [PMID: 8529885] Antoine, R. and Locht, C., Mol. Microbiol. (1992) 6:1785-1799 [PMID: 1321324] |
| Constructed from | pBBR1MCS |
| History | NCCB <- Kovach |
| Score | Ap (100) |
| Transferability | Mobilizable |
| Replicon | REP |
| Copy number | medium |
| Molecular weight | 4,95 |
| Markers | 'lacZalpha |
| Sequence | Genbank U25060 |
| Start codon present | No |
| Remarks on properties | can be mobilized when the RK2 functions are provided in trans, compatible with IncP, IncW, IncQ, ColE1 and p15A replicons |
| Mobility | No |
| Host range | gramnegative vector |
| Usage | general cloning vector |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |
| Comments or additions please to: nccb@cbs.knaw.nl | Date: June 19, 2013 |