| | |
| NCCBNr | 3329 |
| Name | pV1a |
| Element type | Plasmid |
| Collection nrs | PC-V3329 |
| Date of accession | Sep 1992 |
| Deposited by | Van Dijke |
| Distributed in | Escherichia coli K12 JM101 hsdR |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Van Dijke,M.W. et al., Gene (1992) 111:99-104 [PMID: 1547959] |
| Constructed from | pUC19 |
| History | NCCB <- Van Dijke |
| Score | Ap (50) |
| Transferability | Non-transferable |
| Replicon | pMB1 |
| Copy number | high |
| Molecular weight | 2,7 |
| Markers | lacZalpha |
| Promoter | Plac |
| Translation initiation region | Yes |
| Start codon present | Yes |
| Restriction sites | (U,E):AccI,BamHI,EcoRI,HincII,KpnI,PstI,SacI,SalI,SmaI,XbaI,XmaI |
| Mobility | Yes |
| Host range | Escherichia coli vector |
| Usage | general cloning vector |
| Expression vector | heterologous promoter, heterologous translation initiation region, in vitro expression: fusion protein, recombinant protein purification |
| Remarks applications | directs the synthesis of fusion proteins containing an oligo-His domain at the N-terminus. Fusion protein can be purified by immobilized metal affinity chromatography oligo-His domain is expressed in-frame with lacZalpha sequence, allowing color selection of recombinant clones |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |