| | |
| NCCBNr | 3284 |
| Name | pYCplac22 |
| Element type | Plasmid |
| Collection nrs | PC-V3284 |
| Deposited by | Gietz |
| Distributed in | Escherichia coli K12 DH5alpha |
| Distribution restricted | Yes |
| Natural plasmid | No |
| Literature | Gietz, R.D. and Sugino, A., Gene (1988) 74: 527-534 [PMID: 3073106] |
| History | NCCB <- Gietz |
| Score | Ap (50) < E. coli;TRP1 < Yeast |
| Transferability | Non-transferable |
| Replicon | pMB1;ARS1;CEN4 |
| Copy number | high (E. coli);low (Yeast) |
| Molecular weight | 4,85 |
| Markers | lacZ |
| Restriction sites | (U,E,I(lacZ)):BamHI,EcoRI,HindIII,KpnI,PstI,SalI,SmaI,SphI,SstI,XbaI |
| Host range | Escherichia coli vector, yeast vector, shuttle vector |
| Usage | general cloning vector |
| Remarks applications | nucleotide sequence of this plasmid is available on diskette. Information can be obtained from the Phabagen Collection |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |