| | |
| NCCBNr | 3277 |
| Name | pET3c |
| Element type | Plasmid |
| Collection nrs | PC-V3277 |
| Deposited by | Maatman |
| Distributed in | Escherichia coli K12 |
| Distribution restricted | Yes |
| Natural plasmid | No |
| Literature | Rosenberg, A.H. et al., Gene (1987) 56:125-135 [PMID: 3315856]
Studier, F. et al., Meth. Enzymol. (1990) 185:60-89 [PMID: 2199796] |
| Constructed from | pBR322 |
| History | NCCB <- Maatman <- |
| Score | Ap (100) |
| Transferability | Non-transferable |
| Replicon | pMB1 |
| Copy number | high |
| Molecular weight | 4,5 |
| Promoter | pT7phi10 |
| Transcription termination | gene 10 |
| Translation initiation region | gene 10 |
| Start codon present | Yes |
| Restriction sites | (U,E):BamHI,NdeI |
| Remarks on properties | BamHI can be used to produce translational fusions proteins with the first 11 amino-acids of the gene 10 protein; NdeI can be used to fuse sequences directly to the gene 10 codon: this site includes the initiating ATG |
| Host range | Escherichia coli vector |
| Usage | general cloning vector |
| Expression vector | general expression, heterologous promoter |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |