| NCCBNr | 3254 |
| Name | pJF119HE |
| Element type | Plasmid |
| Collection nrs | PC-V3254 |
| Date of accession | Oct 1991 |
| Deposited by | Hekstra |
| Distributed in | Escherichia coli K12 |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Furste, J.P. et al., Gene (1986) 48: 119-131 [PMID: 3549457] |
| Constructed from | pKK223-3 |
| History | NCCB <- Hekstra <- |
| Score | Ap |
| Transferability | Non-transferable |
| Replicon | pMB1 |
| Copy number | high |
| Molecular weight | 5,3 |
| Markers | lacIq |
| Promoter | Ptac |
| Transcription termination | rrnB |
| Translation initiation region | No |
| Start codon present | No |
| Restriction sites | (U,I(bla)):AhaIII,PvuI,ScaI; (U,I(lacIq)):BstEII,HpaI,MluI; (U,E):BamHI,EcoRI,HindII,HindIII,KpnI,PstI,SacI,SalI,SmaI,SphI,XbaI,XmaI |
| Host range | Escherichia coli vector |
| Usage | general cloning vector |
| Expression vector | general expression, heterologous promoter |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |
| Comments or additions please to: nccb@cbs.knaw.nl | Date: May 23, 2013 |