| | |
| NCCBNr | 3243 |
| Name | lambda NK1327 |
| Element type | Phage, Transposon, MiniTransposon |
| Collection nrs | PC-V3243 |
| Date of accession | Jan 1991 |
| Deposited by | Kleckner |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Way et al., Gene (1984) 32:369-379 [PMID: 6099322]
N. Kleckner et al., Methods Enzymol. (1991) 204:139-80 [PMID: 1658561] |
| Constructed from | Pam80 |
| History | NCCB <- Kleckner |
| Host propagation | supE strain (e.g. C600, LE392) |
| Propagation protocol | Sambrook J., Fritsch, E.F. and Maniatis, T., Molecular Cloning, a laboratory manual, 2nd edition, CSH (1989), Chapter 2 |
| Lysogenic | No |
| Plaque forming | No |
| Plaque morphology | No |
| Receptor | LamB |
| Nucleic acid type | DNA, double, linear |
| Markers | ats1 ats2 transposase gene behind Ptac promoter; mini-Tn10 |
| Transposable element | Km-resistance marker from Tn903 and PlacUV5 |
| Host range | Escherichia coli vector |
| Suicide vector | transposon delivery |
| Remarks applications | for generating transposon-fusions of target genes to PlacUV5 |
| Medium | Luria Broth |
| Supply | LS |
| PriceCode | 1 |