| | |
| NCCBNr | 3212 |
| Name | pNK474 |
| Element type | Plasmid |
| Collection nrs | PC-V3212 |
| Date of accession | Jan 1991 |
| Deposited by | Kleckner |
| Distributed in | Escherichia coli K12 JM101 hsdS recA = PC 2495 |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Way et al., Gene (1984) 32:369-379 [PMID: 6099322]
N. Kleckner et al., Methods Enzymol. (1991) 204:139-80 [PMID: 1658561] |
| History | NCCB <- Kleckner |
| Score | Ap |
| Transferability | Non-transferable |
| Replicon | pMB1 |
| Copy number | high |
| Markers | wild-type transposase gene behind Ptac promotor |
| Source of insert | E. coli |
| Markers insert | wild-type transposase gene |
| Production | transposase |
| Host range | Escherichia coli vector |
| Usage | tool plasmid |
| Remarks applications | complements in trans mini-Tn10 carried on vehicles that lack transposase |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |