| | |
| NCCBNr | 3200 |
| Name | pMSEx-2 |
| Element type | Plasmid |
| Collection nrs | PC-V3200 |
| Date of accession | Apr 1991 |
| Deposited by | Schuermann |
| Distributed in | Escherichia coli K12 DH5alpha |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Schuermann, M., Nucl. Acids Res. (1990) 18:4945 [PMID: 2395667] |
| Constructed from | pUC19 |
| History | NCCB <- Schuermann |
| Score | Ap (50);Km (12.5) |
| Remarks propag./selection | cloning efficiencies are substantially better on Ap- plates; Km resistance should be checked at regular intervals (e.g. during amplification steps) |
| Transferability | Non-transferable |
| Replicon | pMB1 |
| Copy number | high |
| Molecular weight | 5,6 |
| Markers | TK-neo |
| Promoter | SV40 early promoter |
| Transcription termination | MSV-LTR fragment (poly-A signal) |
| Translation initiation region | '5-AGCTTGGTACCATGGACCTCGAGGCCTAGCTAGCTAGCTAGGAGCT-3' |
| Start codon present | Yes |
| Restriction sites | (U):BglII; (U,E):StuI |
| Host range | Escherichia coli vector, animal cell vector |
| Usage | general cloning vector |
| Expression vector | general expression, heterologous promoter |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |