| | |
| NCCBNr | 3176 |
| Name | pCL1920 |
| Element type | Plasmid |
| Collection nrs | PC-V3176; DSM 19642 |
| Date of accession | Feb 1991 |
| Deposited by | Lerner |
| Distributed in | Escherichia coli K12 CL83 (JM83-recA56) |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Lerner, C.G. and Inouye, M., Nucl. Acids Res. (1990) 18:4631 [PMID: 2201955] |
| Constructed from | pGB2 |
| History | NCCB <- Lerner |
| Score | Sp (50);Sm (50) |
| Transferability | Non-transferable |
| Replicon | pSC101 |
| Copy number | low |
| Molecular weight | 4,6 |
| Markers | lacZ' |
| Promoter | PlacPO |
| Restriction sites | ():EcoRI; (U,E):BamHI,HindIII,KpnI,PstI,SacI,SalI,SmaI,Xba |
| Remarks on properties | the m.c.s. of pCL1920 is drawn in reverse orientation in Nucl. Acids Res. (1990), 18:4631, please note that pUC19 was used to construct this plasmid |
| Host range | Escherichia coli vector |
| Usage | general cloning vector, export signal probe, for the cloning of potentially toxic genes and the minimalization of expression use pCL1921 |
| Expression vector | general expression, heterologous promoter |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |