| | |
| NCCBNr | 3149 |
| Name | pML133 |
| Element type | Plasmid |
| Collection nrs | PC-V3149 |
| Date of accession | Jan 1991 |
| Deposited by | Labes, M. |
| Distributed in | Escherichia coli K12 DH5 |
| Distribution restricted | No |
| Natural plasmid | No |
| Literature | Labes et al., Gene (1990) 89:37-46 [PMID: 2115488] |
| Constructed from | pML10 |
| History | NCCB <- Labes, M. |
| Score | Gm (10);Sp (100) |
| Remarks propag./selection | selection for plasmid can be done with Sp(100); in E. coli, does not grow on Nm(100), but on Km(25) |
| Incompatibility group | IncQ |
| Transferability | Mobilizable |
| Replicon | p15A;R300B |
| Copy number | medium/high |
| Molecular weight | 11,2 |
| Markers | Nm/Km |
| Promoter | Ptac |
| Restriction sites | (E):EcoRI,PstI,SalI,SmaI; (U):BglII,SacII; (U,E):BamHI,ClaI,HindIII,KpnI,SacI,XbaI,XhoI |
| Host range | gramnegative vector |
| Usage | general cloning vector, transcription termination probe |
| Expression vector | general expression, heterologous promoter |
| Medium | Luria Broth |
| Supply | LY |
| PriceCode | 1 |