Polyphasic identification Help Icon
 
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  • Polyphasic identification parameters
    Minimum similarity to keep identification results (0-100):
    Minimum characters to be accounted :
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Item name  
Number of ascospores per ascus    Go to top of form
Min:LP:HP:Max:

Asci evanescence    Go to top of form
?
evanescent
persistent
evanescent and/or persistent
no asci

Asci shape    Go to top of form
?
not club shaped
club shaped
not club shaped and/or club shaped
no asci

Ascospores with gelatinous sheath    Go to top of form
?
no
yes
no and/or yes
no ascospores

Ascospores with a groove    Go to top of form
?
no
yes
no and/or yes
no ascospores

Basidia septation    Go to top of form
?
one celled
transversely septate
one celled and/or transversely septate
longitudinally-obliquely septate
one celled and/or longitudinally-obliquely septate
transversely septate and/or longitudinally-obliquely septate
all forms possible
no basidia

Basidia shape    Go to top of form
?
clavate
cylindrical
clavate and/or/to cylindrical
capitate
clavate and/or capitate
cylindrical and/or capitate
clavate and/or cylindrical and/or capitate
no basidia

Basidia catenate-solitary    Go to top of form
?
solitary
catenate
solitary and/or catenate
no basidia

Teliospores    Go to top of form
?
no teliospores
angular
angular and/or no teliospores
round to oval
round to oval and/or no teliospores
angular to round to oval
angular to round to oval and/or no teliospores

Sexual reproduction    Go to top of form
?
no sexual reproduction
ascomycetous
basidiomycetous

Ascospores shape    Go to top of form
hat shaped :?  Absent  Variable  Present  
cap shaped :?  Absent  Variable  Present  
saturn shaped :?  Absent  Variable  Present  
walnut shaped :?  Absent  Variable  Present  
conical :?  Absent  Variable  Present  
oblong :?  Absent  Variable  Present  
round :?  Absent  Variable  Present  
oval :?  Absent  Variable  Present  
smooth :?  Absent  Variable  Present  
rough :?  Absent  Variable  Present  
reniform :?  Absent  Variable  Present  
allantoid :?  Absent  Variable  Present  
needle shaped :?  Absent  Variable  Present  
whip like :?  Absent  Variable  Present  

Conjugation    Go to top of form
cell with cell :?  Absent  Variable  Present  
cell with its bud :?  Absent  Variable  Present  
clamp connexions :?  Absent  Variable  Present  
pseudoclamp connexions :?  Absent  Variable  Present  
3.1. Macro-morphology

Reddish diffusing pigment    Go to top of form
?
not produced
produced
produced and/or not produced

Colony margin    Go to top of form
?
entire
eroded
entire and/or eroded
other
entire and/or other
eroded and/or other
entire and/or eroded and/or other

Colony colour    Go to top of form
white :?  Absent  Variable  Present  
cream :?  Absent  Variable  Present  
yellowish :?  Absent  Variable  Present  
orange :?  Absent  Variable  Present  
pink :?  Absent  Variable  Present  
red :?  Absent  Variable  Present  
buff :?  Absent  Variable  Present  
dark brown to black :?  Absent  Variable  Present  

Colony appearance    Go to top of form
arachnoid :?  Absent  Variable  Present  
farinose :?  Absent  Variable  Present  
smooth :?  Absent  Variable  Present  
venose :?  Absent  Variable  Present  
warty :?  Absent  Variable  Present  
furrowed :?  Absent  Variable  Present  

Colony texture    Go to top of form
fluid :?  Absent  Variable  Present  
mucoid :?  Absent  Variable  Present  
butyrous :?  Absent  Variable  Present  
membranous :?  Absent  Variable  Present  

3.2. Micro-mophology

Filaments    Go to top of form
?
no hyphae
pseudohyphae
pseudohyphae and/or no pseudohyphae
septate hyphae
septate hyphae and/or no hyphae
pseudohyphae and/or septate hyphae
septate hyphae, pseudohyphae and/or no hyphae

Asexual reproduction    Go to top of form
fission :?  Absent  Variable  Present  
budding :?  Absent  Variable  Present  
multilateral budding :?  Absent  Variable  Present  
bipolar budding :?  Absent  Variable  Present  
monopolar budding :?  Absent  Variable  Present  
sympodial budding :?  Absent  Variable  Present  
annellidic (percurrent) budding :?  Absent  Variable  Present  
on sterigma :?  Absent  Variable  Present  
on denticles :?  Absent  Variable  Present  
arthroconidia :?  Absent  Variable  Present  
chlamydospores :?  Absent  Variable  Present  
endospores :?  Absent  Variable  Present  
symmetrical ballistoconidia :?  Absent  Variable  Present  
asymmetrical ballistoconidia :?  Absent  Variable  Present  

Cell shape    Go to top of form
lemon :?  Absent  Variable  Present  
triangular :?  Absent  Variable  Present  
lunate :?  Absent  Variable  Present  
allantoid :?  Absent  Variable  Present  
round :?  Absent  Variable  Present  
oval :?  Absent  Variable  Present  
cylindrical :?  Absent  Variable  Present  
fusiform :?  Absent  Variable  Present  
dumb-bell shaped :?  Absent  Variable  Present  
4.1. Fermentation

D-Glucose (F1)    Go to top of form
?
negative
positive
weak, delayed
variable

D-Galactose (F2)    Go to top of form
?
negative
positive
weak, delayed
variable

Maltose (F3)    Go to top of form
?
negative
positive
weak, delayed
variable

Me a-D-Glucoside (F4)    Go to top of form
?
negative
positive
weak, delayed
variable

Sucrose (F5)    Go to top of form
?
negative
positive
weak, delayed
variable

a,a-Trehalose (F6)    Go to top of form
?
negative
positive
weak, delayed
variable

Melibiose (F7)    Go to top of form
?
negative
positive
weak, delayed
variable

Lactose (F8)    Go to top of form
?
negative
positive
weak, delayed
variable

Cellobiose (F9)    Go to top of form
?
negative
positive
weak, delayed
variable

Melezitose (F10)    Go to top of form
?
negative
positive
weak, delayed
variable

Raffinose (F11)    Go to top of form
?
negative
positive
weak, delayed
variable

Inulin (F12)    Go to top of form
?
negative
positive
weak, delayed
variable

Starch (F13)    Go to top of form
?
negative
positive
weak, delayed
variable

D-Xylose (F14)    Go to top of form
?
negative
positive
weak, delayed
variable

4.2. Assimilation-Growth

50% D-Glucose (O4)    Go to top of form
?
no growth
growth
weak, delayed growth
variable

60% D-Glucose (O5)    Go to top of form
?
no growth
growth
weak, delayed growth
variable

Starch formation (M1)    Go to top of form
?
no
yes
weak
variable

Acid acetic production (M2)    Go to top of form
?
no
yes
weak
variable

Urea hydrolysis (M3)    Go to top of form
?
no
yes
weak
variable

Diazonium Blue B reaction (M4)    Go to top of form
?
negative
positive
weak
variable

Methanol (C41)    Go to top of form
?
no growth
growth
weak, delayed growth
variable

Ethanol (C42)    Go to top of form
?
no growth
growth
weak, delayed growth
variable

Yeasts physiological data    Go to top of form
C1 D-Glucose :?  Negative  Variable  Positive  
C2 D-Galactose :?  Negative  Variable  Positive  
C3 L-Sorbose :?  Negative  Variable  Positive  
C4 D-Glucosamine :?  Negative  Variable  Positive  
C5 D-Ribose :?  Negative  Variable  Positive  
C6 D-Xylose :?  Negative  Variable  Positive  
C7 L-Arabinose :?  Negative  Variable  Positive  
C8 D-Arabinose :?  Negative  Variable  Positive  
C9 L-Rhamnose :?  Negative  Variable  Positive  
C10 Sucrose :?  Negative  Variable  Positive  
C11 Maltose :?  Negative  Variable  Positive  
C12 a,a-Trehalose :?  Negative  Variable  Positive  
C13 Me a-D-Glucoside :?  Negative  Variable  Positive  
C14 Cellobiose :?  Negative  Variable  Positive  
C15 Salicin :?  Negative  Variable  Positive  
C16 Arbutin :?  Negative  Variable  Positive  
C17 Melibiose :?  Negative  Variable  Positive  
C18 Lactose :?  Negative  Variable  Positive  
C19 Raffinose :?  Negative  Variable  Positive  
C20 Melezitose :?  Negative  Variable  Positive  
C21 Inulin :?  Negative  Variable  Positive  
C22 Starch :?  Negative  Variable  Positive  
C23 Glycerol :?  Negative  Variable  Positive  
C24 Erythritol :?  Negative  Variable  Positive  
C25 Ribitol :?  Negative  Variable  Positive  
C26 Xylitol :?  Negative  Variable  Positive  
C27 L-Arabinitol :?  Negative  Variable  Positive  
C28 D-Glucitol :?  Negative  Variable  Positive  
C29 D-Mannitol :?  Negative  Variable  Positive  
C30 Galactitol :?  Negative  Variable  Positive  
C31 myo-Inositol :?  Negative  Variable  Positive  
C32 D-Glucono-1,5-lactone :?  Negative  Variable  Positive  
C33 2-Keto-D-Gluconate :?  Negative  Variable  Positive  
C34 5-Keto-D-Gluconate :?  Negative  Variable  Positive  
C35 D-Gluconate :?  Negative  Variable  Positive  
C36 D-Glucuronate :?  Negative  Variable  Positive  
C37 D-Galacturonate :?  Negative  Variable  Positive  
C38 DL-Lactate :?  Negative  Variable  Positive  
C39 Succinate :?  Negative  Variable  Positive  
C40 Citrate :?  Negative  Variable  Positive  
C43 Propane 1,2 diol :?  Negative  Variable  Positive  
C44 Butane 2,3 diol :?  Negative  Variable  Positive  
C45 Quinic acid :?  Negative  Variable  Positive  
C46 D-glucarate :?  Negative  Variable  Positive  
C47 D-Galactonate :?  Negative  Variable  Positive  
C48 Palatinose :?  Negative  Variable  Positive  
C49 Levulinate :?  Negative  Variable  Positive  
C50 L-Malic acid :?  Negative  Variable  Positive  
C51 L-Tartaric acid :?  Negative  Variable  Positive  
C52 D-Tartaric acid :?  Negative  Variable  Positive  
C53 meso-Tartaric acid :?  Negative  Variable  Positive  
C54 Galactaric acid :?  Negative  Variable  Positive  
C55 Uric acid :?  Negative  Variable  Positive  
C56 Gentobiose :?  Negative  Variable  Positive  
C57 Ethylene glycol :?  Negative  Variable  Positive  
C58 Tween 40 :?  Negative  Variable  Positive  
C59 Tween 60 :?  Negative  Variable  Positive  
C60 Tween 80 :?  Negative  Variable  Positive  
N1 Nitrate :?  Negative  Variable  Positive  
N2 Nitrite :?  Negative  Variable  Positive  
N3 Ethylamine :?  Negative  Variable  Positive  
N4 L-Lysine :?  Negative  Variable  Positive  
N5 Cadaverine :?  Negative  Variable  Positive  
N6 Creatine :?  Negative  Variable  Positive  
N7 Creatinine :?  Negative  Variable  Positive  
N8 Glucosamine :?  Negative  Variable  Positive  
N9 Imidazole :?  Negative  Variable  Positive  
N10 D-Tryptophan :?  Negative  Variable  Positive  
N11 D-Proline :?  Negative  Variable  Positive  
N12 Putrescine :?  Negative  Variable  Positive  
V1 w/o vitamins :?  Negative  Variable  Positive  
V2 w/o myo-Inositol :?  Negative  Variable  Positive  
V3 w/o Pantothenate :?  Negative  Variable  Positive  
V4 w/o Biotin :?  Negative  Variable  Positive  
V5 w/o Thiamin :?  Negative  Variable  Positive  
V6 w/o Biotin & Thiamin :?  Negative  Variable  Positive  
V7 w/o Pyridoxine :?  Negative  Variable  Positive  
V8 w/o Pyridoxine & Thiamin :?  Negative  Variable  Positive  
V9 w/o Niacin :?  Negative  Variable  Positive  
V10 w/o PABA :?  Negative  Variable  Positive  
O1 Cycloheximide 0.01% :?  Negative  Variable  Positive  
O2 Cycloheximide 0.1% :?  Negative  Variable  Positive  
O3 Acetic acid 1% :?  Negative  Variable  Positive  
O6 10% NaCl :?  Negative  Variable  Positive  
O7 16% NaCl :?  Negative  Variable  Positive  
O8 Growth at pH=3 :?  Negative  Variable  Positive  
O9 Growth at pH=9.5 :?  Negative  Variable  Positive  
O10 Fluconazole :?  Negative  Variable  Positive  

4.3. CoQ

Coenzyme Q system    Go to top of form

4.4. Temperatures

Temperatures growths tests     Go to top of form
at 4ºC :?  Absent  Variable  Present  
at 12ºC :?  Absent  Variable  Present  
at 15ºC :?  Absent  Variable  Present  
at 18ºC :?  Absent  Variable  Present  
at 21ºC :?  Absent  Variable  Present  
at 25ºC :?  Absent  Variable  Present  
at 30ºC :?  Absent  Variable  Present  
at 35ºC :?  Absent  Variable  Present  
at 37ºC :?  Absent  Variable  Present  
at 40ºC :?  Absent  Variable  Present  
at 42ºC :?  Absent  Variable  Present  
at 45ºC :?  Absent  Variable  Present  
5.1. Other information

5.2. Sequences

rDNA sequences 26S (Paste sequence):  Click me to open a help window on this field  Go to top of form

rDNA sequences 18S (Paste sequence):  Click me to open a help window on this field  Go to top of form

rDNA sequences ITS (Paste sequence):  Click me to open a help window on this field  Go to top of form

5.3. Electrophoresis
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