Aspergillus and Penicillium

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- Pairwise sequence alignment (use CBS database as reference)
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Polyphasic identification

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Polyphasic identification parameters

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1. Description
2. Macro-morphology
3. Micro-morphology
4. DNA sequences

Item name

2.1. Data CYAS at 25C

Colony diameter CYAS (mm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Reverse colour on CYAS25C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYAS25C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.2. Data CYA at 30C

Colony diameter CYA30C (mm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Reverse colour on CYA30C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA30C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.3. Data CYA at 37C

Colony diameter CYA37C (mm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Reverse colour on CYA37C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA37C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.4. Data MEA at 25C

Reverse colour on MEA Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on MEA Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Colony diameter MEA (mm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

2.5. Data CREA at 25C

Acid production on CREA Go to top of form

Go to top of form

None
Weak
Moderate
Good
Huge
None to huge
None, moderate and/or huge
?

Base production on CREA Go to top of form

Go to top of form

No base production
Poor base production OR delayed
Base production
?

2.6. Data CYA at 25C

Ehrlich reaction Go to top of form

Go to top of form

no reaction :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
violet :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present

Reverse colour on CYA Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Colony diameter CYA (mm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

3.1. Stipe

Stipe ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
warted :	?	Absent	Variable	Present
rugose :	?	Absent	Variable	Present

Stipe length (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Conidiophore seriation Go to top of form

Go to top of form

uniseriate
uniseriate or biseriate
biseriate
?

3.4. Apical vesicle

Apical vesicle width (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Apical vesicle shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
subclavate :	?	Absent	Variable	Present
clavate :	?	Absent	Variable	Present
pyriform :	?	Absent	Variable	Present
flask-shaped (ampulliform :	?	Absent	Variable	Present
irregular :	?	Absent	Variable	Present
domelike :	?	Absent	Variable	Present
conical :	?	Absent	Variable	Present
spatulate :	?	Absent	Variable	Present

Apical vesicle Go to top of form

Go to top of form

absent
variable
present
?

Apical vesicle length (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Stipe width (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Branching pattern Go to top of form

Go to top of form

Mono-verticillate :	?	Absent	Variable	Present
Bi-verticillate :	?	Absent	Variable	Present
Ter-verticillate :	?	Absent	Variable	Present
Quater-verticillate :	?	Absent	Variable	Present
Multi-verticillate :	?	Absent	Variable	Present

3.2. Phialide

Phialide shape Go to top of form

Go to top of form

acerose (lanceolate, without constriction) :	?	Absent	Variable	Present
flask-shaped, (ampulliform, with constriction) :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present

3.3. Conidia

Conidia shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
ellipsoid :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present
barrel-shaped :	?	Absent	Variable	Present
ovoid :	?	Absent	Variable	Present
pyriform :	?	Absent	Variable	Present
citriform :	?	Absent	Variable	Present
apiculate :	?	Absent	Variable	Present

Conidia wall thickness Go to top of form

Go to top of form

thin-walled
variable
thick-walled
?

Conidia length (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Conidia width (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Conidia in situ Go to top of form

Go to top of form

in columns
variable
radiate
?

Conidia ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
warted :	?	Absent	Variable	Present
echinulate :	?	Absent	Variable	Present
spinulose :	?	Absent	Variable	Present
striate :	?	Absent	Variable	Present

3.4. Sexual features

Cleistothecia or sclerotia Go to top of form

Go to top of form

absent
variable
present
?

Cleistothecia/sclerotia colour Go to top of form

Go to top of form

white to creamy :	?	Absent	Variable	Present
buff :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
purple :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
gray :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
greenish :	?	Absent	Variable	Present

Cleistothecia surface cells Go to top of form

Go to top of form

hyphoid
variable
parenchymatous
?

Number of ascospores Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Ascospore shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
ellipsoid :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present
ovoid :	?	Absent	Variable	Present
lenticular :	?	Absent	Variable	Present

Ascospore ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
echinulate :	?	Absent	Variable	Present
spinulose :	?	Absent	Variable	Present
striate, with ridges (here not lenticular!) :	?	Absent	Variable	Present
reticulate :	?	Absent	Variable	Present
rugulose :	?	Absent	Variable	Present

Ascospore lenticular Go to top of form

Go to top of form

absent :	?	Absent	Variable	Present
with a single flange :	?	Absent	Variable	Present
with 2 flanges :	?	Absent	Variable	Present
with 3 flanges :	?	Absent	Variable	Present
with 4 flanges :	?	Absent	Variable	Present
multiple flangs :	?	Absent	Variable	Present
no flanges, but a furrow (groove) :	?	Absent	Variable	Present

Ascospore length (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Ascospore width (µm) Go to top of form

Go to top of form

Min:

LP:

HP:

Max:

Hülle cells Go to top of form

Go to top of form

no
variable
yes
?

Elongation factor (Paste sequence):

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Go to top of form

LSU sequences (Paste sequence):

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Go to top of form

SSU sequences (Paste sequence):

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Go to top of form

ITS sequences (Paste sequence):

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Beta-tubulin from Genbank (Paste sequence):

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Go to top of form

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