Penicillium

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Identification
- Pairwise sequence alignment (use CBS database as reference)
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Polyphasic identification

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Polyphasic identification parameters

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1. Description
2. Micro-morphology
3. Macro-morphology
4. DNA sequences

Item name

2.1. Phialide

Phialide length (µm) Go to top of form

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Min:

Max:

2.2. Metulae

Metulae length (µm) Go to top of form

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Min:

Max:

2.3. Conidia

Conidia length (µm) Go to top of form

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Min:

Max:

Conidia width (µm) Go to top of form

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Min:

Max:

Roughness conidia Go to top of form

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Smooth
Smooth
Rough walled
Smooth
Spinose
Smooth to spinose
Smooth
Smooth, rough and/or spinose
Smooth

2.4. Stipe

Stipe width (µm) Go to top of form

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Min:

Max:

Roughness stipe Go to top of form

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Smooth
Smooth
Obviously rough, but not distinct
Smooth
Warted
Smooth and/or warted
Smooth
Smooth, rough and/or warted
Smooth

2.5. Pattern conidiophore

Branching pattern Go to top of form

Go to top of form

Mono-verticillate :	?	Absent	Variable	Present
Bi-verticillate :	?	Absent	Variable	Present
Ter-verticillate :	?	Absent	Variable	Present
Quater-verticillate :	?	Absent	Variable	Present

Adpressedness Go to top of form

Go to top of form

Strongly adpressed
Strongly adpressed
Not adpressed nor divergent
Strongly adpressed
Strongly divergent
Strongly adpressed to strongly divergent
Strongly adpressed
Not adpressed to adpressed to divergent
Strongly adpressed

3.1. Data CREA

Acid production on CREA Go to top of form

Go to top of form

None
None
Moderate
None
Huge
None to huge
None
None, moderate and/or huge
None

Base production on CREA Go to top of form

Go to top of form

No base production
No base production
Base production
No base production
No base production
No base production

Degree of growth on CREA Go to top of form

Go to top of form

No or very weak growth
No or very weak growth
Moderate growth
No or very weak growth
Good growth
No growth and/or good growth
No or very weak growth
No, moderate and/or good growth
No or very weak growth

Colony diameter CREA (mm) Go to top of form

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Min:

Max:

3.2. Data YES

Sporulation on YES Go to top of form

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No sporulation
No sporulation
Moderate sporulation
No sporulation
Strong sporulation
No sporulation and/or strong sporulation
No sporulation
No, moderate and/or strong sporulation
No sporulation

Reverse colour on YES Go to top of form

Go to top of form

Pale :	?	Absent	Variable	Present
Yellow :	?	Absent	Variable	Present
Orange :	?	Absent	Variable	Present
Red :	?	Absent	Variable	Present
Beige, light brown, creme brown :	?	Absent	Variable	Present
Brown :	?	Absent	Variable	Present
Dark brown/blackish green :	?	Absent	Variable	Present

Colony diameter YES (mm) Go to top of form

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Min:

Max:

3.3. Data MEA

Fasiculation on MEA Go to top of form

Go to top of form

Velvety
Velvety
Weakly fasiculate
Velvety
Strongly fasiculate or coremiform
Velvety and/or strongly fasciculate
Velvety
Velvety, weakly and/or strongly fasciculate
Velvety

Lanose on MEA Go to top of form

Go to top of form

Velvety
Velvety
Weakly lanose
Velvety
Strongly lanose / floccose
Velvety and/or strongly lanose/floccose
Velvety
Velvety, weakly and/or strongly lanose/floccose
Velvety

Colony diameter MEA (mm) Go to top of form

Go to top of form

Min:

Max:

3.4. Data CYA

Reverse colour on CYA Go to top of form

Go to top of form

Pale :	?	Absent	Variable	Present
Yellow :	?	Absent	Variable	Present
Orange :	?	Absent	Variable	Present
Red :	?	Absent	Variable	Present
Beige, light brown, creme brown :	?	Absent	Variable	Present
Brown :	?	Absent	Variable	Present
Dark brown / blackish green :	?	Absent	Variable	Present

Conidium colour on CYA Go to top of form

Go to top of form

Blue green :	?	Absent	Variable	Present
Dark green :	?	Absent	Variable	Present
Dull green :	?	Absent	Variable	Present
Grey green :	?	Absent	Variable	Present
Olive green :	?	Absent	Variable	Present
Yellow/pure green :	?	Absent	Variable	Present
White :	?	Absent	Variable	Present

Ehrlich reaction Go to top of form

Go to top of form

No reaction or Yellow
No reaction or Yellow
Pink (to red) reaction
No reaction or Yellow
Violet reaction
Intermediate state between violet and yellow/no reaction
No reaction or Yellow
No reaction, yellow, pink, red and/or violet
No reaction or Yellow

Colony diameter CYA (mm) Go to top of form

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Min:

Max:

3.5. Ecophysiological data

Ratio CYAS/CYA Go to top of form

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Min:

Max:

Ratio CYA15/CYA25 Go to top of form

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Min:

Max:

Ratio CYA30/CYA25 Go to top of form

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Min:

Max:

3.6. Data CZ

Colony diameter CZ (mm) Go to top of form

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Min:

Max:

3.7. Data DG18

Colony diameter DG18 (mm) Go to top of form

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Min:

Max:

Partial beta-tubulin (Paste sequence): Go to top of form

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Beta-tubulin from Genbank (Paste sequence): Go to top of form

Go to top of form

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