| Authors : | Diez,B.;Marcos,A.T.;Rodriguez,M.;de la Fuente,J.L.;Barredo,J.L. | |
| Keywords : | GENBANK/AF056975
5' Untranslated Regions
Actins/*genetics
Cloning, Molecular
Gene Dosage
*Genes, Fungal
Molecular Sequence Data
Penicillium chrysogenum/classification/*genetics
Phylogeny
Sequence Analysis, DNA
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| Remarks : | The nucleotide sequence of a 2994-bp genomic fragment, including the gamma-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript. | |