Polyphasic identification Help Icon
 
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  • Polyphasic identification parameters
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Item name  


Colony texture    Go to top of form  
fluid:?  No  Variable  Yes  
mucoid:?  No  Variable  Yes  
butyrous:?  No  Variable  Yes  
membranous:?  No  Variable  Yes  

Colony appearance    Go to top of form  
arachnoid:?  No  Variable  Yes  
farinose:?  No  Variable  Yes  
smooth:?  No  Variable  Yes  
venose:?  No  Variable  Yes  
warty:?  No  Variable  Yes  
furrowed:?  No  Variable  Yes  

Colony colour    Go to top of form  
white:?  No  Variable  Yes  
cream:?  No  Variable  Yes  
yellowish:?  No  Variable  Yes  
orange:?  No  Variable  Yes  
pink:?  No  Variable  Yes  
red:?  No  Variable  Yes  
buff:?  No  Variable  Yes  
dark brown to black:?  No  Variable  Yes  

Colony margin    Go to top of form  
?
entire
eroded
entire and/or eroded
other
entire and/or other
eroded and/or other
entire and/or eroded and/or other

Reddish diffusing pigment    Go to top of form  
?
not produced
produced
produced and/or not produced

Filaments    Go to top of form  
?
no hyphae
pseudohyphae
pseudohyphae and/or no pseudohyphae
septate hyphae
septate hyphae and/or no hyphae
pseudohyphae and/or septate hyphae
septate hyphae, pseudohyphae and/or no hyphae

Cell shape    Go to top of form  
lemon:?  No  Variable  Yes  
triangular:?  No  Variable  Yes  
lunate:?  No  Variable  Yes  
allantoid:?  No  Variable  Yes  
round:?  No  Variable  Yes  
oval:?  No  Variable  Yes  
cylindrical:?  No  Variable  Yes  
fusiform:?  No  Variable  Yes  
dumb-bell shaped:?  No  Variable  Yes  

Asexual reproduction    Go to top of form  
fission:?  No  Variable  Yes  
budding:?  No  Variable  Yes  
multilateral budding:?  No  Variable  Yes  
bipolar budding:?  No  Variable  Yes  
monopolar budding:?  No  Variable  Yes  
sympodial budding:?  No  Variable  Yes  
annellidic (percurrent) budding:?  No  Variable  Yes  
on sterigma:?  No  Variable  Yes  
on denticles:?  No  Variable  Yes  
arthroconidia:?  No  Variable  Yes  
chlamydospores:?  No  Variable  Yes  
endospores:?  No  Variable  Yes  
symmetrical ballistoconidia:?  No  Variable  Yes  
asymmetrical ballistoconidia:?  No  Variable  Yes  

Conjugation    Go to top of form  
cell with cell:?  No  Variable  Yes  
cell with its bud:?  No  Variable  Yes  
clamp connexions:?  No  Variable  Yes  
pseudoclamp connexions:?  No  Variable  Yes  

Teliospores    Go to top of form  
?
no teliospores
angular
angular and/or no teliospores
round to oval
round to oval and/or no teliospores
angular to round to oval
angular to round to oval and/or no teliospores

Asci shape    Go to top of form  
?
not club shaped
club shaped
not club shaped and/or club shaped
no asci

Asci evanescence    Go to top of form  
?
evanescent
persistent
evanescent and/or persistent
no asci

Ascospores shape    Go to top of form  
hat shaped:?  No  Variable  Yes  
cap shaped:?  No  Variable  Yes  
saturn shaped:?  No  Variable  Yes  
walnut shaped:?  No  Variable  Yes  
conical:?  No  Variable  Yes  
oblong:?  No  Variable  Yes  
round:?  No  Variable  Yes  
oval:?  No  Variable  Yes  
smooth:?  No  Variable  Yes  
rough:?  No  Variable  Yes  
reniform:?  No  Variable  Yes  
allantoid:?  No  Variable  Yes  
needle shaped:?  No  Variable  Yes  
whip like:?  No  Variable  Yes  

Ascospores with gelatinous sheath    Go to top of form  
?
no
yes
no and/or yes
no ascospores

Ascospores with a groove    Go to top of form  
?
no
yes
no and/or yes
no ascospores

Basidia shape    Go to top of form  
?
clavate
cylindrical
clavate and/or/to cylindrical
capitate
clavate and/or capitate
cylindrical and/or capitate
clavate and/or cylindrical and/or capitate
no basidia

Basidia catenate-solitary    Go to top of form  
?
solitary
catenate
solitary and/or catenate
no basidia

Basidia septation    Go to top of form  
?
one celled
transversely septate
one celled and/or transversely septate
longitudinally-obliquely septate
one celled and/or longitudinally-obliquely septate
transversely septate and/or longitudinally-obliquely septate
all forms possible
no basidia

Fermentations

D-Glucose (F1)    Go to top of form  
?
-
+
-, +

D-Galactose (F2)    Go to top of form  
?
-
+
-, +

Maltose (F3)    Go to top of form  
?
-
+
-, +

Me a-D-Glucoside (F4)    Go to top of form  
?
-
+
-, +

Sucrose (F5)    Go to top of form  
?
-
+
-, +

a,a-Trehalose (F6)    Go to top of form  
?
-
+
-, +

Melibiose (F7)    Go to top of form  
?
-
+
-, +

Lactose (F8)    Go to top of form  
?
-
+
-, +

Cellobiose (F9)    Go to top of form  
?
-
+
-, +

Melezitose (F10)    Go to top of form  
?
-
+
-, +

Raffinose (F11)    Go to top of form  
?
-
+
-, +

Inulin (F12)    Go to top of form  
?
-
+
-, +

Starch (F13)    Go to top of form  
?
-
+
-, +

D-Xylose (F14)    Go to top of form  
?
-
+
-, +

Assimilations & growth tests

Assimilations    Go to top of form  
Glucose:?  Negative  Variable  Positive  
Inulin:?  Negative  Variable  Positive  
Sucrose:?  Negative  Variable  Positive  
Raffinose:?  Negative  Variable  Positive  
Melibiose:?  Negative  Variable  Positive  
Galactose:?  Negative  Variable  Positive  
Lactose:?  Negative  Variable  Positive  
Trehalose:?  Negative  Variable  Positive  
Maltose:?  Negative  Variable  Positive  
Melezitose:?  Negative  Variable  Positive  
Methyl-α-D-glucoside:?  Negative  Variable  Positive  
Soluble starch:?  Negative  Variable  Positive  
Cellobiose:?  Negative  Variable  Positive  
Salicin:?  Negative  Variable  Positive  
L-Sorbose:?  Negative  Variable  Positive  
L-Rhamnose:?  Negative  Variable  Positive  
D-Xylose:?  Negative  Variable  Positive  
L-Arabinose:?  Negative  Variable  Positive  
D-Arabinose:?  Negative  Variable  Positive  
D-Ribose:?  Negative  Variable  Positive  
Methanol:?  Negative  Variable  Positive  
Ethanol:?  Negative  Variable  Positive  
Glycerol:?  Negative  Variable  Positive  
Erythritol:?  Negative  Variable  Positive  
Ribitol:?  Negative  Variable  Positive  
Galactitol:?  Negative  Variable  Positive  
D-Mannitol:?  Negative  Variable  Positive  
D-Glucitol:?  Negative  Variable  Positive  
myo-Inositol:?  Negative  Variable  Positive  
DL-Lactate:?  Negative  Variable  Positive  
Succinate:?  Negative  Variable  Positive  
Citrate:?  Negative  Variable  Positive  
D-Gluconate:?  Negative  Variable  Positive  
D-Glucosamine:?  Negative  Variable  Positive  
N-Acetyl-D-glucosamine:?  Negative  Variable  Positive  
Hexadecane:?  Negative  Variable  Positive  
Nitrate:?  Negative  Variable  Positive  
Nitrite:?  Negative  Variable  Positive  
Vitamin-free:?  Negative  Variable  Positive  
2-Keto-D-gluconate:?  Negative  Variable  Positive  
5-Keto-D-gluconate:?  Negative  Variable  Positive  
Saccharate:?  Negative  Variable  Positive  
Xylitol:?  Negative  Variable  Positive  
L-Arabinitol:?  Negative  Variable  Positive  
Arbutin:?  Negative  Variable  Positive  
Propane 1,2 diol:?  Negative  Variable  Positive  
Butane 2,3 diol:?  Negative  Variable  Positive  
Cadaverine:?  Negative  Variable  Positive  
Creatinine:?  Negative  Variable  Positive  
L-Lysine:?  Negative  Variable  Positive  
Ethylamine:?  Negative  Variable  Positive  
50% Glucose:?  Negative  Variable  Positive  
10% NaCl/5% glucose:?  Negative  Variable  Positive  
Starch formation:?  Negative  Variable  Positive  
Urease:?  Negative  Variable  Positive  
Gelatin liquefaction:?  Negative  Variable  Positive  
Cycloheximide 0.01%:?  Negative  Variable  Positive  
Cycloheximide 0.1%:?  Negative  Variable  Positive  

Diazonium Blue B reaction (M4)    Go to top of form  
?
-
+
-, +

Coenzyme Q system    Go to top of form  

rDNA sequences 26S (Paste sequence):    Go to top of form  

rDNA sequences ITS (Paste sequence):    Go to top of form