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General information

 
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 NCCB number :NCCB 3000 
 Element type :Plasmid 
 Other culture collection nrs :PC-V3000 
 Distributed in :Escherichia coli K12 HB101 
 Restricted distribution :No 
 Naturally occurring :No 
 Literature :O'Connor, C.D. et al., Gene 20 (1982) 219-229 
 Scorable phenotype :Ap; Cm 
 Transfer ability :Non-transferable 
 Copy number :pMB1 
 Molecular weight (in kb) :6.1 
 Remarks on properties :cells carrying this plasmid have to be grown at 30 C; expression of the EcoRI gene is lethal to the cells at higher temperatures; recombinants do survive at 37 C 
 Vector host range :Escherichia coli vector 
 Supply :LY 
 Lysogenic :No 
 Plaque forming phage :No 
 Clear plaque morphology :No 
 Unique restriction sites :AvaI; BglII(EcoRI); EcoRI; HindIII(EcoRI) 
 Price :150 
 
Biosafety and restrictions
 GMO :Yes 

Media and growth conditions

 Medium :