Aspergillus and Penicillium

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- Pairwise sequence alignment (use CBS database as reference)
- Polyphasic identification on: 'Aspergillus and Penicillium Species'
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Polyphasic identification

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Polyphasic identification parameters

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1. Description
2. Macro-morphology
3. Micro-morphology
4. DNA sequences

Item name

2.1. Data CYAS at 25C

Colony diameter CYAS (mm) Go to top of form

Go to top of form

Min:

Max:

Reverse colour on CYAS25C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYAS25C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.2. Data CYA at 30C

Colony diameter CYA30C (mm) Go to top of form

Go to top of form

Min:

Max:

Reverse colour on CYA30C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA30C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.3. Data CYA at 37C

Colony diameter CYA37C (mm) Go to top of form

Go to top of form

Min:

Max:

Reverse colour on CYA37C Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA37C Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

2.4. Data MEA at 25C

Reverse colour on MEA Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on MEA Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Colony diameter MEA (mm) Go to top of form

Go to top of form

Min:

Max:

2.5. Data CREA at 25C

Acid production on CREA Go to top of form

Go to top of form

None
None
Moderate
None
Huge
None to huge
None
None, moderate and/or huge
None

Base production on CREA Go to top of form

Go to top of form

No base production
No base production
Base production
No base production
No base production
No base production

2.6. Data CYA at 25C

Ehrlich reaction Go to top of form

Go to top of form

no reaction :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
violet :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present

Reverse colour on CYA Go to top of form

Go to top of form

unchanged :	?	Absent	Variable	Present
white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Conidium colour on CYA Go to top of form

Go to top of form

white to cream :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
red :	?	Absent	Variable	Present
olive :	?	Absent	Variable	Present
green :	?	Absent	Variable	Present
fawn to pale brown :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
grey or beige :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
blue :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
glaucous :	?	Absent	Variable	Present

Colony diameter CYA (mm) Go to top of form

Go to top of form

Min:

Max:

3.1. Stipe

Stipe ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
warted :	?	Absent	Variable	Present
rugose :	?	Absent	Variable	Present

Stipe length (µm) Go to top of form

Go to top of form

Min:

Max:

Conidiophore seriation Go to top of form

Go to top of form

uniseriate
uniseriate
biseriate
uniseriate
uniseriate
uniseriate

3.4. Apical vesicle

Apical vesicle width (µm) Go to top of form

Go to top of form

Min:

Max:

Apical vesicle shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
subclavate :	?	Absent	Variable	Present
clavate :	?	Absent	Variable	Present
pyriform :	?	Absent	Variable	Present
flask-shaped (ampulliform :	?	Absent	Variable	Present
irregular :	?	Absent	Variable	Present
domelike :	?	Absent	Variable	Present
conical :	?	Absent	Variable	Present
spatulate :	?	Absent	Variable	Present

Apical vesicle Go to top of form

Go to top of form

absent
absent
present
absent
absent
absent

Apical vesicle length (µm) Go to top of form

Go to top of form

Min:

Max:

Stipe width (µm) Go to top of form

Go to top of form

Min:

Max:

Branching pattern Go to top of form

Go to top of form

Mono-verticillate :	?	Absent	Variable	Present
Bi-verticillate :	?	Absent	Variable	Present
Ter-verticillate :	?	Absent	Variable	Present
Quater-verticillate :	?	Absent	Variable	Present
Multi-verticillate :	?	Absent	Variable	Present

3.2. Phialide

Phialide shape Go to top of form

Go to top of form

acerose (lanceolate, without constriction) :	?	Absent	Variable	Present
flask-shaped, (ampulliform, with constriction) :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present

3.3. Conidia

Conidia shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
ellipsoid :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present
barrel-shaped :	?	Absent	Variable	Present
ovoid :	?	Absent	Variable	Present
pyriform :	?	Absent	Variable	Present
citriform :	?	Absent	Variable	Present
apiculate :	?	Absent	Variable	Present

Conidia wall thickness Go to top of form

Go to top of form

thin-walled
thin-walled
thick-walled
thin-walled
thin-walled
thin-walled

Conidia length (µm) Go to top of form

Go to top of form

Min:

Max:

Conidia width (µm) Go to top of form

Go to top of form

Min:

Max:

Conidia in situ Go to top of form

Go to top of form

in columns
in columns
radiate
in columns
in columns
in columns

Conidia ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
warted :	?	Absent	Variable	Present
echinulate :	?	Absent	Variable	Present
spinulose :	?	Absent	Variable	Present
striate :	?	Absent	Variable	Present

3.4. Sexual features

Cleistothecia or sclerotia Go to top of form

Go to top of form

absent
absent
present
absent
absent
absent

Cleistothecia/sclerotia colour Go to top of form

Go to top of form

white to creamy :	?	Absent	Variable	Present
buff :	?	Absent	Variable	Present
yellow :	?	Absent	Variable	Present
orange :	?	Absent	Variable	Present
purple :	?	Absent	Variable	Present
black :	?	Absent	Variable	Present
gray :	?	Absent	Variable	Present
brown :	?	Absent	Variable	Present
pink :	?	Absent	Variable	Present
greenish :	?	Absent	Variable	Present

Cleistothecia surface cells Go to top of form

Go to top of form

hyphoid
hyphoid
parenchymatous
hyphoid
hyphoid
hyphoid

Number of ascospores Go to top of form

Go to top of form

Min:

Max:

Ascospore shape Go to top of form

Go to top of form

globose :	?	Absent	Variable	Present
subglobose :	?	Absent	Variable	Present
ellipsoid :	?	Absent	Variable	Present
cylindrical :	?	Absent	Variable	Present
ovoid :	?	Absent	Variable	Present
lenticular :	?	Absent	Variable	Present

Ascospore ornamentation Go to top of form

Go to top of form

smooth :	?	Absent	Variable	Present
finely roughened :	?	Absent	Variable	Present
coarsely roughened :	?	Absent	Variable	Present
echinulate :	?	Absent	Variable	Present
spinulose :	?	Absent	Variable	Present
striate, with ridges (here not lenticular!) :	?	Absent	Variable	Present
reticulate :	?	Absent	Variable	Present
rugulose :	?	Absent	Variable	Present

Ascospore lenticular Go to top of form

Go to top of form

absent :	?	Absent	Variable	Present
with a single flange :	?	Absent	Variable	Present
with 2 flanges :	?	Absent	Variable	Present
with 3 flanges :	?	Absent	Variable	Present
with 4 flanges :	?	Absent	Variable	Present
multiple flangs :	?	Absent	Variable	Present
no flanges, but a furrow (groove) :	?	Absent	Variable	Present

Ascospore length (µm) Go to top of form

Go to top of form

Min:

Max:

Ascospore width (µm) Go to top of form

Go to top of form

Min:

Max:

Hülle cells Go to top of form

Go to top of form

no
no
yes
no
no
no

Elongation factor (Paste sequence):

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Go to top of form

LSU sequences (Paste sequence):

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Go to top of form

SSU sequences (Paste sequence):

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Go to top of form

ITS sequences (Paste sequence):

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Go to top of form

Beta-tubulin from Genbank (Paste sequence):

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Go to top of form

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